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insertions in a similar coding sequence. This gene encodes a protein belonging to the family of periplasmic chaperones involved in pilus assembly via the so-known as chaperone/usher pathway (31). These chaperones are periplasmic proteins that adopt an Ig-like fold for recognition and even further assembly of pilin subunits (32). Floor exposure necessary the functionality of a next accessory component, termed usher, that varieties a channel in the outer membrane through which the pilin subunits are sequentially assembled (33).
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To show slot online that The full chaperone/usher pathway is associated with biofilm formation, and the nonadherent phenotype isn't exclusively connected to a defect in the CupA2 chaperone, we introduced a mutation from the gene encoding the usher component (
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) along with the attachment phenotype was analyzed as Beforehand described. In distinction to PAN67B, neither PAB3B nor PAC3B was altered within their capability to sort a biofilm in microtiter dishes.
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